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Presterilized with 70% ethanol. Elutriation was performed at a rotor speed of 2,200 rpm (683×g) and temperature of 20◦ C for 4–5 h. Subpopulations of cells were eluted in F12 medium containing 1% FBS. ) (23). 0 elutriation chamber is used with the Avanti J-25 and Avanti J-26 XP series and with J6-MI centrifuges equipped with viewport door and strobe assembly, accelerated throughput, shorter run times can be achieved. 0 elutriation system. The protocol presented here has been developed to enable cell cycle studies in a variety of cell lines (25–27).